Bovine aortic endothelial cells (BAEC), detached from thoracic aorta by collagenase treatment, have been studied after adding a mixture of glycosaminoglycans (GAGs) (the composition of which has been studied by Casu et al. -6), at different concentrations. GAGs at the concentration of 90 mg/100 ml of medium (Dulbecco's modification of Eagle's MEM with 10% foetal calf serum) produced an increase in the growth speed. We have quantitized these data both by counting the number of cells 24 h after trypsinization in phase-contrast-microscopy, and by measuring the time necessary to reach a certain number of influences. Ultrastructural observation at transmission electron microscope after freeze-etching preparation, didn't show any relevant modification either in the organization of cell membrane or in the cytoplasmatic structures. An increase of "vesicular openings" ("pits") on the plasma-membrane was evident.
Weber, G., Bianciardi, G., Giacchi, M., Toti, P., Vatti, R., Weber, E., et al. (1982). On the growth of cultured bovine aortic endothelial cells. Preliminary observations of ultrastructural (freeze-etching) morphology and quantitation of growth speed at phase-contrast microscopic examination: Effect of glycosaminoglycans (GAGs) [Sull' accrescimento in coltura di cellule endoteliali di aorta bovina. Osservazioni preliminari di morfologia ultrastrutturale al crio-decappaggio e quantizzazione della velocita' di crescita in microscopia a contrasto di fase: effetto di glicosaminoglicani]. BOLLETTINO DELLA SOCIETA' ITALIANA DI BIOLOGIA SPERIMENTALE, 58(21), 1380-1386.
On the growth of cultured bovine aortic endothelial cells. Preliminary observations of ultrastructural (freeze-etching) morphology and quantitation of growth speed at phase-contrast microscopic examination: Effect of glycosaminoglycans (GAGs) [Sull' accrescimento in coltura di cellule endoteliali di aorta bovina. Osservazioni preliminari di morfologia ultrastrutturale al crio-decappaggio e quantizzazione della velocita' di crescita in microscopia a contrasto di fase: effetto di glicosaminoglicani]
Bianciardi, Giorgio;Giacchi, M.;Toti, P.;Weber, Elisabetta;
1982-01-01
Abstract
Bovine aortic endothelial cells (BAEC), detached from thoracic aorta by collagenase treatment, have been studied after adding a mixture of glycosaminoglycans (GAGs) (the composition of which has been studied by Casu et al. -6), at different concentrations. GAGs at the concentration of 90 mg/100 ml of medium (Dulbecco's modification of Eagle's MEM with 10% foetal calf serum) produced an increase in the growth speed. We have quantitized these data both by counting the number of cells 24 h after trypsinization in phase-contrast-microscopy, and by measuring the time necessary to reach a certain number of influences. Ultrastructural observation at transmission electron microscope after freeze-etching preparation, didn't show any relevant modification either in the organization of cell membrane or in the cytoplasmatic structures. An increase of "vesicular openings" ("pits") on the plasma-membrane was evident.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/11365/20168
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