Objective[s]: Activin A and inhibin A are growth factors expressed by human endometrium involved in the control of endometrial functions. In the present study we investigated the effects of activin A and inhibin A in modulating the tumor necrosis factor (TNF)-α/intercellular adhesion molecule (ICAM)-1 system in cultured human endometrial stromal cells. Study design: Endometrial samples were obtained from 34 reproductive age women undergoing laparoscopy for benign ovarian cysts or infertility. Endometrial stromal cells were cultured and soluble ICAM-1 and TNF-α were measured in cell-free supernatants following treatment with or without activin A or inhibin A. Cell surface ICAM-1 was assayed by flow cytometry by staining endometrial cells with specific monoclonal antibodies. Results: Activin A and inhibin A did not influence either the expression of cell surface ICAM-1 or soluble ICAM-1 shedding by cultured endometrial cells. On the other hand, TNF-α secretion significantly increased in presence of activin A but not of inhibin A. Conclusions: Since TNF-α modulates several endometrial processes such as menstruation, proliferation, apoptosis, implantation and decidualization, an effect of activin A in the physiological control of endometrium is further supported by the present data. © 2005 Elsevier Ireland Ltd. All rights reserved.

Mangioni, S., Vigan, P., Florio, P., Borghi, O., Vignali, M., Petraglia, F., et al. (2005). Effect of activin A on tumor necrosis factor-alpha/intercellular adhesion molecule-1 pathway in endometrial stromal cells. EUROPEAN JOURNAL OF OBSTETRICS, GYNECOLOGY, AND REPRODUCTIVE BIOLOGY, 123(2), 218-223 [10.1016/j.ejogrb.2005.03.017].

Effect of activin A on tumor necrosis factor-alpha/intercellular adhesion molecule-1 pathway in endometrial stromal cells

FLORIO, P.;
2005-01-01

Abstract

Objective[s]: Activin A and inhibin A are growth factors expressed by human endometrium involved in the control of endometrial functions. In the present study we investigated the effects of activin A and inhibin A in modulating the tumor necrosis factor (TNF)-α/intercellular adhesion molecule (ICAM)-1 system in cultured human endometrial stromal cells. Study design: Endometrial samples were obtained from 34 reproductive age women undergoing laparoscopy for benign ovarian cysts or infertility. Endometrial stromal cells were cultured and soluble ICAM-1 and TNF-α were measured in cell-free supernatants following treatment with or without activin A or inhibin A. Cell surface ICAM-1 was assayed by flow cytometry by staining endometrial cells with specific monoclonal antibodies. Results: Activin A and inhibin A did not influence either the expression of cell surface ICAM-1 or soluble ICAM-1 shedding by cultured endometrial cells. On the other hand, TNF-α secretion significantly increased in presence of activin A but not of inhibin A. Conclusions: Since TNF-α modulates several endometrial processes such as menstruation, proliferation, apoptosis, implantation and decidualization, an effect of activin A in the physiological control of endometrium is further supported by the present data. © 2005 Elsevier Ireland Ltd. All rights reserved.
2005
Mangioni, S., Vigan, P., Florio, P., Borghi, O., Vignali, M., Petraglia, F., et al. (2005). Effect of activin A on tumor necrosis factor-alpha/intercellular adhesion molecule-1 pathway in endometrial stromal cells. EUROPEAN JOURNAL OF OBSTETRICS, GYNECOLOGY, AND REPRODUCTIVE BIOLOGY, 123(2), 218-223 [10.1016/j.ejogrb.2005.03.017].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/19371
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