We have used electron spin resonance (ESR) techniques to study the binding of divalent cations to diphtheria toxin (DT). Addition of DT to Mn(II) solution at a stoichiometry of 2:1 (DT:Mn) induces a 79% loss in the intensity of the ESR spectrum of Mn(II) suggesting a strong binding of Mn(II) to DT. Inclusion of Ca(II) at a ratio of 1:2:1 (Ca:DT:Mn) in the reaction mixture restores the intensity of the Mn(II) signal to 64%. This indicates that Ca(II) and Mn(II) share same binding site(s) in DT. The results presented in this communication suggest that DT is a Ca(II) binding protein. © 1986 Academic Press, Inc.
Basosi, R., Kushnaryov, V., Panz, T., Lai, C.S. (1986). Evidence that divalent cations bind to diphteria toxin: an ESR approach. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 139(3), 991-995 [10.1016/S0006-291X(86)80275-3].
Evidence that divalent cations bind to diphteria toxin: an ESR approach
Basosi, Riccardo;
1986-01-01
Abstract
We have used electron spin resonance (ESR) techniques to study the binding of divalent cations to diphtheria toxin (DT). Addition of DT to Mn(II) solution at a stoichiometry of 2:1 (DT:Mn) induces a 79% loss in the intensity of the ESR spectrum of Mn(II) suggesting a strong binding of Mn(II) to DT. Inclusion of Ca(II) at a ratio of 1:2:1 (Ca:DT:Mn) in the reaction mixture restores the intensity of the Mn(II) signal to 64%. This indicates that Ca(II) and Mn(II) share same binding site(s) in DT. The results presented in this communication suggest that DT is a Ca(II) binding protein. © 1986 Academic Press, Inc.
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https://hdl.handle.net/11365/17538
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