The complex-formation equilibria of the pentadecapeptide TLEGTKKGHKLHLDY, the 114-128 protein fragment of SPARC, with the Cull ion have been investigated, at I = 0.1 mol.dm(-3) (KNO3) and T = 298.2 K. Protonation and complex-formation constants have been determined potentiometrically, and formation enthalpies measured by direct solution calorimetry; the complex-formation model and species stoichiometry have been carefully checked by means of UV/Vis absorption, CD and EPR spectroscopy. The structure hypo-theses of the complex species are also based on detailed study of the H-1 and C-13 NMR spectra of the ligand in both the absence and presence of copper ions. The involvement in complex-formation of both the terminal amino and imidazole groups has been suggested and their specific behaviour at different pH values elucidated.

Conato, C., Kamysz, W., Kozlowski, H., Luczkowski, M., Mackiewicz, Z., Mancini, F., et al. (2003). Cu(II) ion coordination to unprotected pentadecapeptide contaning two His residues: competition between the terminal amino and the side-chain imidazole nitrogen donors. EUROPEAN JOURNAL OF INORGANIC CHEMISTRY(9), 1694-1702 [10.1002/ejic.200200551].

Cu(II) ion coordination to unprotected pentadecapeptide contaning two His residues: competition between the terminal amino and the side-chain imidazole nitrogen donors

VALENSIN, DANIELA;VALENSIN, GIANNI
2003

Abstract

The complex-formation equilibria of the pentadecapeptide TLEGTKKGHKLHLDY, the 114-128 protein fragment of SPARC, with the Cull ion have been investigated, at I = 0.1 mol.dm(-3) (KNO3) and T = 298.2 K. Protonation and complex-formation constants have been determined potentiometrically, and formation enthalpies measured by direct solution calorimetry; the complex-formation model and species stoichiometry have been carefully checked by means of UV/Vis absorption, CD and EPR spectroscopy. The structure hypo-theses of the complex species are also based on detailed study of the H-1 and C-13 NMR spectra of the ligand in both the absence and presence of copper ions. The involvement in complex-formation of both the terminal amino and imidazole groups has been suggested and their specific behaviour at different pH values elucidated.
Conato, C., Kamysz, W., Kozlowski, H., Luczkowski, M., Mackiewicz, Z., Mancini, F., et al. (2003). Cu(II) ion coordination to unprotected pentadecapeptide contaning two His residues: competition between the terminal amino and the side-chain imidazole nitrogen donors. EUROPEAN JOURNAL OF INORGANIC CHEMISTRY(9), 1694-1702 [10.1002/ejic.200200551].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/12121
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