CD4 engagement triggers an early signaling cascade which initiates late events such as transcription factor activation. The outcome of CD4 engagement is T-cell commitment to alternative, dramatically different fates, such as activation and apoptosis. We have tested a panel of anti-CD4 mAbs specific for different CD4 epitopes, as well as HIV-1 gp120, for the capacity to activate crucial early events such as enhancement of p561ck kinase activity and Shc phosphoryation. The same CD4 epitopes were characterized for their capacity both to deliver a gene activating signal and to program T-cells to activation dependent death. No correlation could be found between capacity of specific CD4 epitopes to deliver a gene activating signal and capacity to prime T-cells to apoptotis, suggesting that gene activating and proapoptotic potential are independent functions of CD4 epitopes. Furthermore, while triggering of the calcium pathway appears critical in NF-AT activation, optimal p561ck activation and Shc phosphorylation might be required for initiation of the apoptotic pathway.

Milia, E., DI SOMMA, M.M., Majolini, M.B., Ulivieri, C., Somma, C., Piccolella, E., et al. (1997). Gene activating and proapoptotic potential are independent properties of different CD4 epitopes. MOLECULAR IMMUNOLOGY, 34(4), 287-296 [10.1016/S0161-5890(97)00050-3].

Gene activating and proapoptotic potential are independent properties of different CD4 epitopes

ULIVIERI, CRISTINA;BALDARI, C. T.
1997-01-01

Abstract

CD4 engagement triggers an early signaling cascade which initiates late events such as transcription factor activation. The outcome of CD4 engagement is T-cell commitment to alternative, dramatically different fates, such as activation and apoptosis. We have tested a panel of anti-CD4 mAbs specific for different CD4 epitopes, as well as HIV-1 gp120, for the capacity to activate crucial early events such as enhancement of p561ck kinase activity and Shc phosphoryation. The same CD4 epitopes were characterized for their capacity both to deliver a gene activating signal and to program T-cells to activation dependent death. No correlation could be found between capacity of specific CD4 epitopes to deliver a gene activating signal and capacity to prime T-cells to apoptotis, suggesting that gene activating and proapoptotic potential are independent functions of CD4 epitopes. Furthermore, while triggering of the calcium pathway appears critical in NF-AT activation, optimal p561ck activation and Shc phosphorylation might be required for initiation of the apoptotic pathway.
1997
Milia, E., DI SOMMA, M.M., Majolini, M.B., Ulivieri, C., Somma, C., Piccolella, E., et al. (1997). Gene activating and proapoptotic potential are independent properties of different CD4 epitopes. MOLECULAR IMMUNOLOGY, 34(4), 287-296 [10.1016/S0161-5890(97)00050-3].
File in questo prodotto:
File Dimensione Formato  
Gene activating and proapoptotic potential are independent properties of different CD4 epitopes.pdf

non disponibili

Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 1.21 MB
Formato Adobe PDF
1.21 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/11513
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo