We have introduced protein thiolation index (PTI), i.e. the molar ratio of the sum of all low molecular mass thiols bound to plasma proteins to protein free cysteinyl residues, as a sensitive biomarker of oxidative stress. According to the original procedure its determination requires a rapid separation of plasma and a specific treatment of samples to stabilize thiols. Here we demonstrate that samples can be collected without use of any anticoagulant to prevent blood clotting and without any stabilization of thiols too. This simplification of the determination of PTI makes its analysis more feasible also in routine clinical laboratories.

Giustarini, D., Galvagni, F., Colombo, G., Dalle-Donne, I., Milzani, A., Aloisi, A.M., et al. (2017). Determination of protein thiolation index (PTI) as a biomarker of oxidative stress in human serum. ANALYTICAL BIOCHEMISTRY, 538, 38-41 [10.1016/j.ab.2017.09.010].

Determination of protein thiolation index (PTI) as a biomarker of oxidative stress in human serum

Giustarini, Daniela;Galvagni, Federico;Aloisi, Anna Maria;Rossi, Ranieri
2017-01-01

Abstract

We have introduced protein thiolation index (PTI), i.e. the molar ratio of the sum of all low molecular mass thiols bound to plasma proteins to protein free cysteinyl residues, as a sensitive biomarker of oxidative stress. According to the original procedure its determination requires a rapid separation of plasma and a specific treatment of samples to stabilize thiols. Here we demonstrate that samples can be collected without use of any anticoagulant to prevent blood clotting and without any stabilization of thiols too. This simplification of the determination of PTI makes its analysis more feasible also in routine clinical laboratories.
2017
Giustarini, D., Galvagni, F., Colombo, G., Dalle-Donne, I., Milzani, A., Aloisi, A.M., et al. (2017). Determination of protein thiolation index (PTI) as a biomarker of oxidative stress in human serum. ANALYTICAL BIOCHEMISTRY, 538, 38-41 [10.1016/j.ab.2017.09.010].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/1035280