Engagement of the T cell antigen receptor (TCR) by specific ligand bound to the major histocompatibility complex is the primary event that leads to the assembly of the immune synapse (IS). Central to this process is TCR clustering at the T cell-APC contact, which is achieved with the contribution of an endosomal pool that is delivered to the IS by polarized recycling. As the TCR recycling process has not been fully elucidated, we developed methods suitable to quantitate recycling to the plasma membrane of TCR/CD3 complexes that have been engaged at the cell surface and track their traffic through the intracellular vesicular compartments toward the IS.

Patrussi, L., Baldari, C. (2017). Analysis of TCR/CD3 recycling at the immune synapse. In The Immune Synapse. (pp. 143-155). New York : Humana Press [10.1007/978-1-4939-6881-7_10].

Analysis of TCR/CD3 recycling at the immune synapse

Patrussi, Laura;Baldari, Cosima
2017-01-01

Abstract

Engagement of the T cell antigen receptor (TCR) by specific ligand bound to the major histocompatibility complex is the primary event that leads to the assembly of the immune synapse (IS). Central to this process is TCR clustering at the T cell-APC contact, which is achieved with the contribution of an endosomal pool that is delivered to the IS by polarized recycling. As the TCR recycling process has not been fully elucidated, we developed methods suitable to quantitate recycling to the plasma membrane of TCR/CD3 complexes that have been engaged at the cell surface and track their traffic through the intracellular vesicular compartments toward the IS.
2017
978-1-4939-6879-4
978-1-4939-6881-7
Patrussi, L., Baldari, C. (2017). Analysis of TCR/CD3 recycling at the immune synapse. In The Immune Synapse. (pp. 143-155). New York : Humana Press [10.1007/978-1-4939-6881-7_10].
File in questo prodotto:
File Dimensione Formato  
Chapter 10.pdf

non disponibili

Tipologia: PDF editoriale
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 336.4 kB
Formato Adobe PDF
336.4 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/1006266